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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been observed in many tumors, which leads to chromosome instability, and so may possibly contribute to tumorigenesis. This protein was found to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a important position in advertising mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been explained. Two extra splice variants were being documented but detailed nucleotide sequence facts just isn't yet offered.
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Blocking peptides are peptides that bind particularly to your target antibody and block antibody binding. These peptide typically incorporates the epitope recognized with the antibody.
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This guidebook outlines additional possible optimization of mobile and immunoassay parameters to be sure the best possible results are...
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This tutorial outlines further more probable optimization of mobile and immunoassay parameters to be certain the very best success are...
Antibodies bound to the blocking peptide now not bind towards the epitope about the goal protein. By comparing the staining in the blocked antibody versus the antibody alone, you can see which staining is precise.
Focus on Data Cyclin E belongs on the very conserved cyclin loved ones, whose members are characterised by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation styles which contribute into the temporal coordination of every mitotic occasion. Cyclin E forms a posh with and features as a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and is also degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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